Demo 02 of 11
Sequencing read QC and trimming
Synthetic data for illustration
- What it shows
- Per-position base quality before and after Trimmomatic-style trimming, with adjustable window size, quality threshold, and minimum length, plus example reads and the surviving length distribution.
- How it maps to my work
- The first stage of my M.Tech RNA-seq pipeline used Trimmomatic before HISAT2 alignment. It also draws on the DTU course on whole genome sequencing tools. See M.Tech thesis.
- Data source
- Synthetic data for illustration.